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calcein am pi double staining kit  (Beyotime)


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    Structured Review

    Beyotime calcein am pi double staining kit
    Calcein Am Pi Double Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 3025 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am/Calcein+AM/pmc12926989-407-9-12
    Average 99 stars, based on 3025 article reviews
    calcein am pi double staining kit - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    other:


    Incubation:

    Article Title: A novel photosensitizer-based photodynamic therapy reprograms the Kynurenine–AhR axis to boost antitumor immunity in breast cancer
    Article Snippet: .. After an additional 24 h incubation, 4T1 cells were stained using a Calcein AM and propidium iodide (PI) double-staining kit (C2015S, Beyotime Biotechnology) to distinguish live and dead cells following the manufacturer's protocol. ..

    Article Title: Gypenosides inhibit melanoma proliferation, migration and enhance the anti-tumor immunity of CD8 + T cells via ETV5/PD-L1 signaling.
    Article Snippet: .. First of all, B16F10-OVA cells were incubated with Calcein-AM (green fluorescence, #C2012, Beyotime, China) for 30 min. Then, the B16F10-OVA cells were mixed with the corresponding proportion of OT-I cells in the T cell culture medium for co-culture, and incubated at 37 °C with 5 % CO2 for 8 h. Then, the staining results were observed under a fluorescence microscope (Leica DMi8, Germany) and photographed. ..

    Staining:

    Article Title: A novel photosensitizer-based photodynamic therapy reprograms the Kynurenine–AhR axis to boost antitumor immunity in breast cancer
    Article Snippet: .. After an additional 24 h incubation, 4T1 cells were stained using a Calcein AM and propidium iodide (PI) double-staining kit (C2015S, Beyotime Biotechnology) to distinguish live and dead cells following the manufacturer's protocol. ..

    Article Title: 3D-printing flexible PLGA scaffold modified with bioorthogonal IGF-1 for skin regeneration
    Article Snippet: .. On day 4 and 7, in order to evaluate cell adhesion, Calcein AM (Beyotime) was used to stain the cells. ..

    Article Title: Gypenosides inhibit melanoma proliferation, migration and enhance the anti-tumor immunity of CD8 + T cells via ETV5/PD-L1 signaling.
    Article Snippet: .. First of all, B16F10-OVA cells were incubated with Calcein-AM (green fluorescence, #C2012, Beyotime, China) for 30 min. Then, the B16F10-OVA cells were mixed with the corresponding proportion of OT-I cells in the T cell culture medium for co-culture, and incubated at 37 °C with 5 % CO2 for 8 h. Then, the staining results were observed under a fluorescence microscope (Leica DMi8, Germany) and photographed. ..

    Double Staining:

    Article Title: A novel photosensitizer-based photodynamic therapy reprograms the Kynurenine–AhR axis to boost antitumor immunity in breast cancer
    Article Snippet: .. After an additional 24 h incubation, 4T1 cells were stained using a Calcein AM and propidium iodide (PI) double-staining kit (C2015S, Beyotime Biotechnology) to distinguish live and dead cells following the manufacturer's protocol. ..

    Transfection:

    Article Title: NDST3 suppression restores lysosomal acidification and ameliorates amyloid-β and MAPT/tau pathology in Alzheimer's disease.
    Article Snippet: .. Forty-eight hours after the transfection of shRNA, the cells were subjected to cell survival assay using calcein AM (#C2012, Beyotime) according to the manufacturer’s instructions. ..

    Article Title: NDST3 suppression restores lysosomal acidification and ameliorates amyloid-β and MAPT/tau pathology in Alzheimer’s disease
    Article Snippet: .. Forty-eight hours after the transfection of shRNA, the cells were subjected to cell survival assay using calcein AM (#C2012, Beyotime) according to the manufacturer’s instructions. ..

    shRNA:

    Article Title: NDST3 suppression restores lysosomal acidification and ameliorates amyloid-β and MAPT/tau pathology in Alzheimer's disease.
    Article Snippet: .. Forty-eight hours after the transfection of shRNA, the cells were subjected to cell survival assay using calcein AM (#C2012, Beyotime) according to the manufacturer’s instructions. ..

    Article Title: NDST3 suppression restores lysosomal acidification and ameliorates amyloid-β and MAPT/tau pathology in Alzheimer’s disease
    Article Snippet: .. Forty-eight hours after the transfection of shRNA, the cells were subjected to cell survival assay using calcein AM (#C2012, Beyotime) according to the manufacturer’s instructions. ..

    Clonogenic Cell Survival Assay:

    Article Title: NDST3 suppression restores lysosomal acidification and ameliorates amyloid-β and MAPT/tau pathology in Alzheimer's disease.
    Article Snippet: .. Forty-eight hours after the transfection of shRNA, the cells were subjected to cell survival assay using calcein AM (#C2012, Beyotime) according to the manufacturer’s instructions. ..

    Article Title: NDST3 suppression restores lysosomal acidification and ameliorates amyloid-β and MAPT/tau pathology in Alzheimer’s disease
    Article Snippet: .. Forty-eight hours after the transfection of shRNA, the cells were subjected to cell survival assay using calcein AM (#C2012, Beyotime) according to the manufacturer’s instructions. ..

    Saline:

    Article Title: Ameliorating post-infarction myocardial fibrosis and cardiac function via ROS-responsive hydrogel-mediated IL-11 antibody delivery
    Article Snippet: .. According to the manufacturer's instructions (Beyotime, China), Calcein AM and propidium iodide (PI) were diluted to final concentrations of 2 μM and 8 μM, respectively, using Dulbecco's phosphate-buffered saline (DPBS). ..

    Fluorescence:

    Article Title: Gypenosides inhibit melanoma proliferation, migration and enhance the anti-tumor immunity of CD8 + T cells via ETV5/PD-L1 signaling.
    Article Snippet: .. First of all, B16F10-OVA cells were incubated with Calcein-AM (green fluorescence, #C2012, Beyotime, China) for 30 min. Then, the B16F10-OVA cells were mixed with the corresponding proportion of OT-I cells in the T cell culture medium for co-culture, and incubated at 37 °C with 5 % CO2 for 8 h. Then, the staining results were observed under a fluorescence microscope (Leica DMi8, Germany) and photographed. ..

    Cell Culture:

    Article Title: Gypenosides inhibit melanoma proliferation, migration and enhance the anti-tumor immunity of CD8 + T cells via ETV5/PD-L1 signaling.
    Article Snippet: .. First of all, B16F10-OVA cells were incubated with Calcein-AM (green fluorescence, #C2012, Beyotime, China) for 30 min. Then, the B16F10-OVA cells were mixed with the corresponding proportion of OT-I cells in the T cell culture medium for co-culture, and incubated at 37 °C with 5 % CO2 for 8 h. Then, the staining results were observed under a fluorescence microscope (Leica DMi8, Germany) and photographed. ..

    Microscopy:

    Article Title: Gypenosides inhibit melanoma proliferation, migration and enhance the anti-tumor immunity of CD8 + T cells via ETV5/PD-L1 signaling.
    Article Snippet: .. First of all, B16F10-OVA cells were incubated with Calcein-AM (green fluorescence, #C2012, Beyotime, China) for 30 min. Then, the B16F10-OVA cells were mixed with the corresponding proportion of OT-I cells in the T cell culture medium for co-culture, and incubated at 37 °C with 5 % CO2 for 8 h. Then, the staining results were observed under a fluorescence microscope (Leica DMi8, Germany) and photographed. ..



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    Image Search Results


    ERO1L induces macrophage infiltration. A IHC staining showing F4/80 + , CD86 + , and CD206 + macrophage infiltration in the KC and KEC pancreas tissues harvested from the recovery phase. Scale bar: 50 μm. B In the recovery phase, F4/80 + , CD86 + , and CD206 + macrophage infiltration in the DMSO-treated or EN460-treated KC pancreas tissues were analyzed by IHC method. Scale bar: 50 μm. C Analyzing the correlation between ERO1L expression and various immune cells using the TCGA database. D Representative IHC staining images of ERO1L and CD68 in the pancreatic cancer specimens from Ren Ji cohort, scale bar: 100 μm. The correlation curve shows the correlation between ERO1L H-score and macrophage marker (CD68) in pancreatic cancer tissues. E Chemotaxis assay and statistical results of human macrophages (THP-1) treated with conditioned medium from sh-ERO1L and shCtrl pancreatic cancer cells (Capan-2 and MiaPaCa-2). **** P < 0.0001

    Journal: Cell & Bioscience

    Article Title: ERO1L induces macrophage infiltration to potentiate Kras G12D and inflammation-induced pancreatic tumorigenesis via oxidative protein folding of CCL2

    doi: 10.1186/s13578-026-01601-3

    Figure Lengend Snippet: ERO1L induces macrophage infiltration. A IHC staining showing F4/80 + , CD86 + , and CD206 + macrophage infiltration in the KC and KEC pancreas tissues harvested from the recovery phase. Scale bar: 50 μm. B In the recovery phase, F4/80 + , CD86 + , and CD206 + macrophage infiltration in the DMSO-treated or EN460-treated KC pancreas tissues were analyzed by IHC method. Scale bar: 50 μm. C Analyzing the correlation between ERO1L expression and various immune cells using the TCGA database. D Representative IHC staining images of ERO1L and CD68 in the pancreatic cancer specimens from Ren Ji cohort, scale bar: 100 μm. The correlation curve shows the correlation between ERO1L H-score and macrophage marker (CD68) in pancreatic cancer tissues. E Chemotaxis assay and statistical results of human macrophages (THP-1) treated with conditioned medium from sh-ERO1L and shCtrl pancreatic cancer cells (Capan-2 and MiaPaCa-2). **** P < 0.0001

    Article Snippet: Following a 12-h incubation of the THP-1 cells, the migrated cells were treated with 2 μM Calcein-AM (MCE, HY-D0041) for 15 min and subsequently observed under a fluorescence microscope.

    Techniques: Immunohistochemistry, Expressing, Marker, Chemotaxis Assay